Parasite Can Flip a Biological Switch and Keep Going Without Oxygen
A single-celled amoeba linked to contact lens eye infections and blindness keeps going even when its oxygen is cut off. According to a new study in the journal Cell, Acanthamoeba appears to pull it off by switching on a backup system that releases hydrogen gas. In lab tests, it survived at least five days with no oxygen at all.
Acanthamoeba turns up around the world and can cause painful, infectious blindness and fatal brain abscesses. It lives in places as different as air-exposed soil and deep water with almost no oxygen. Low-oxygen pockets are expected inside the body too, in abscesses and in the cornea, especially during sleep or behind contact lenses, which are a known risk factor for the eye infection. Current drugs are toxic and poorly targeted, and surgery is frequently needed.
Scientists had suspected for years that this amoeba carried unusual oxygen-free machinery, but nobody had shown it making hydrogen. A team from Massachusetts General Hospital, Harvard Medical School, the Broad Institute, and Boston University set out to settle that. Most known mitochondria, the structures that power cells, either depend on oxygen, as in humans, or run entirely without it, as in some parasites. This amoeba’s mitochondria can do both, switching modes depending on how much oxygen is around.
Acanthamoeba Mitochondria Hold 381 Proteins With No Obvious Human or Yeast Match
Before testing what the machinery could do, researchers needed a complete parts list. They tagged the outside of the amoeba’s mitochondria with a glowing marker and used magnetic beads to pull the structures out intact. Each sample was then analyzed to identify its proteins, with statistical checks to screen out contaminants from other parts of the cell.
Microscopy added a second check. Of 76 proteins tagged and tracked, 74 landed inside the mitochondria, backing up the larger list. The final catalog holds 1,122 proteins. Of those, 741 have clear counterparts in human or yeast mitochondria, while 381 have no obvious match in either. By the team’s estimate, about 8 percent of the proteins identified through lab measurements may not belong, compared with roughly 25 percent in the raw data of an earlier 2014 effort.
Nearly All Acanthamoeba Mitochondrial Proteins Increased When Oxygen Dropped
Next, researchers grew amoeba cells under 15 different conditions, varying oxygen from normal air to almost none and changing the nutrients too. They expected most of the mitochondrial machinery to power down without oxygen, with a handful of backup proteins switching on. Instead, nearly all mitochondrial proteins increased as oxygen fell.
A specific group tied to oxygen-free energy production climbed the most, including an enzyme called HydA that can generate hydrogen gas. The cells also changed shape and lost their grip on the surface without oxygen, then returned to normal within an hour of air coming back. Without oxygen, starved amoebas could not form the tough protective shells called cysts. The authors speculate that the amoeba’s two-mode mitochondria may resemble those of the last common ancestor of animals, plants, fungi, and amoebas, a point that bears on a long-running debate over whether early mitochondria relied on oxygen.
Source : https://studyfinds.com/blinding-eye-amoeba-secret-survival-trick/